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. 2020 Jan 14;11:264. doi: 10.1038/s41467-019-13839-2

Fig. 2. Macrophages in the DRG, but not at the nerve injury site, are required for mechanical allodynia initiation.

Fig. 2

ae Effect of SNI followed by cannula injection with VEH or AP on immunostaining of GFP+ macrophages at the peripheral nerve (PN) ligature site (ac) and DRG (d) (n = 3 per group) and on mechanical thresholds (e). Dashed lines in a and b denote ligature site; arrows point proximally. Scale bar: 50 µm. Gray shading indicates injection days. Data presented as mean ± SEM. Student’s t-test in c and d, and two-way repeated-measures ANOVA with Sidak’s correction in e. ***P < 0.001, NS nonsignificant compared with control. Source data are available as a Source Data file.