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. 2020 Jan 14;10:245. doi: 10.1038/s41598-019-56386-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Erythrocyte invasion history of parasite cultures used for primary analyses. Efficiency of invasion into neuraminidase treated erythrocytes by all biological replicates of static cultured baseline parasites (week 0) and their respective suspended cultures were monitored at regular intervals by invasion assays. Baseline cultures were confirmed to be SA-dependent, while suspended parasites gradually acquired SA-independent invasion phenotype with increasing length of time in culture. Schizonts were harvested at baseline, and weeks 8 and 6, respectively for suspended W2mef (a) and Dd2 (b). Broken lines indicate suspended replicates which were not sequenced.