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. 2020 Jan 14;10:245. doi: 10.1038/s41598-019-56386-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Baseline and static cultures have similar gene expression patterns in comparison to suspended W2mef. Differential expression analyses were conducted for baseline vs suspended (baseline; vertical axis) and then static vs suspended (static; horizontal axis). A spearman rank correlation plot comparing the differential gene expression between baseline and suspended culture (vertical axis) as against static and suspended cultures (horizontal axis) was then constructed using the log2 fold change values from each comparison. ρ is the coefficient of correlation. Red dotted lines indicate the significance threshold which was set at 4-fold increase in gene expression.