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. 2020 Jan 14;10:245. doi: 10.1038/s41598-019-56386-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pattern of differential gene expression is similar between Dd2 and W2mef. (a) Differential gene expression analyses between baseline and suspended Dd2 shows increased expression of Rh4, eba165 in addition to other genes to be highly expressed in suspended Dd2. Grey: Benjamini-Hochberg adjusted P value < 0.01, red: absolute log2 fold change >1. Genes with symbols have no known function. Black: Benjamini-Hochberg adjusted P value > 0.01 (considered not significant). (b) A Spearman rank correlation plot of differential gene expression between suspended vs baseline W2mef (horizontal axis) and suspended vs baseline of Dd2 (vertical axis) shows similarities in the expression pattern between suspended cultures of the two strains. ρ is the coefficient of correlation.