Table 1.

Comparison of methodologies for metabolic flux analysis.

Requirements	13C-metabolic flux analysis (13C-MFA)	Isotopomer analysis (Conventional)	Isotopomer analysis (This study)
1. (Pseudo) metabolic and isotopically steady state	Required	Required	Required
2. Specific rates data	Required	Not required	Not required
3. In silico metabolic model	Required for finding best-fitted metabolic flux	Not required	Required for the Metropolis-Hastings algorithm
4. Number of data points	Larger than degree of freedom of in silico metabolic model	No restriction	Larger number is preferable
5. Statistical estimation of significance	95% confidence interval of point estimation result	Not evaluated	Cohen’s effect size, d