Fig. 4. Bach2 is intrinsically required for pTreg cell differentiation.

a Flow cytometry plots and quantification showing GATA3, RORγt and Helios expression by Treg cells from the colonic lamina propria of Bach2^fl/flCd4^Cre and control mice. b Flow cytometry plots showing Foxp3 expression by wildtype and Bach2^−/− CD4 T cells after three days in Treg cell-inducing culture conditions, and frequency of Foxp3-expressing cells (right). c Histograms showing Blimp1-GFP expression by Blimp1^GFP and Bach2^−/−Blimp1^GFP CD4 T cells after culture in Treg cell-inducing conditions. Dashed line indicates background fluorescence levels in non-reporter cells. d Flow cytometry plots showing Foxp3 and IL-10 expression by wildtype and Bach2^−/− CD4 T cells after Treg cell-inducing culture, and quantification (right). e Flow cytometry plots showing Foxp3 expression by wildtype, Bach2^−/−, Blimp1^fl/flLck^Cre and Bach2^−/−Blimp1^fl/flLck^Cre after three days culture in Treg cell-inducing conditions, and frequency of Foxp3 and IL-10 expressing cells (below). Flow cytometry plots are representative, data pooled from (a) or representative of two (c–e) or six (b) independent experiments. Statistical significance tested using two-way ANOVA with Tukey’s test (e). Otherwise, significance was tested using the unpaired Student’s t-test. Error bars denote mean ± S.D. Source data are provided as a Source Data file.