Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Schematic expression and purification protocol, including typical times for performance. Yields of NT*-Aβ42/40 are derived from 1 L expression cultures and extrapolated from purification from 100 and 500 mL, resulting in very similar values. (B) SDS-PAGE gel, with protein ladder (L), void (V), before (I) and after SEC yielding pure Aβ. An uncropped full-length gel is presented in Supplementary Fig. S2. (C) SEC diagram showing separation of TEV, NT* and monomeric Aβ.
