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. 2020 Jan 14;10:235. doi: 10.1038/s41598-019-57143-x

Table 2.

Yields of Aβ40 and Aβ42 variants reported in literature and herein.

Aβ variant Fusion partner/expression method Purified Aβ peptide yield in mg/L in rich medium Purified Aβ peptide yield in mg/L in minimal medium Reference
Aβ(1–40) NT*FlSp 40 ± 5 13 ± 4 here
(NANP)19 22 22
IFABP 4 24
GST 7 1.5 23
Aβ(M1–40) Directly from inclusion bodies 10–20 21
Directly from inclusion bodies 10–15 51
Co-expression with ZAβ3 4 20
Aβ(1–42) NT*FlSp 37 ± 7 14 ± 2 here
(NANP)19 19 22
IFABP 3 24
IFABP 6 52
GST 15 53
Ub 4 54
Aβ(M1–42) Directly from inclusion bodies 8 21
Co-expression with ZAβ3 3 20