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. Author manuscript; available in PMC: 2020 Jan 14.
Published in final edited form as: DNA Repair (Amst). 2019 Mar 7;77:76–86. doi: 10.1016/j.dnarep.2019.03.004

Fig. 5. Levels of AFB1-induced DNA adducts in liver DNA from of WT and Neil1−/− mice following AFB1 IP injection.

Fig. 5.

Neil1−/− and control WT mice (6 day old pups) were injected with 10 mM AFB1 in DMSO at a dose of 3.5 mg/kg. Livers were harvested at 6 and 48 h post-injection, immediately frozen in liquid nitrogen, and DNA isolated for AFB1 adduct analysis. Following acid hydrolysis, internal 15N5-guanine-derived standards were added to permit quantitative analysis by isotope dilution mass spectrometry for both AFB1-N7-dG and AFB1-Fapy-dG. Adducts were separated by ultra-performance liquid-chromatography mass spectrometry. The protonated parent ion of the AFB1-N7-dG adduct (m/z 480.1) was selected and subjected to collision-induced fragmentation producing a m/z 152 product ion that was monitored to quantify adduct levels. The AFB1-Fapy-dG adduct was measured by selection of the m/z 498 parent ion and monitoring the collision-induced product ion m/z 452.29. [Reproduced from [76], Fig. 2].