Figure 1.
Screening of immune responses against a panel of predicted neo-antigen candidates reveals oligoreactivity. a. Representative ELISPOT wells showing IFNγ production in response to HA; UQCRC2 wild type (WT) and mutant (MUT) peptides; WT and MUT neo-antigen peptide pools. b. Dot plot summarizing responses against individual peptides, and peptide pools in AB1 murine mesothelioma bearing mice. Group A: 17 peptides, Group B: 16 peptides. (n = 8–16, 3 separate experiments). c.Responses against peptide pools of 5–6 neo-antigen candidates in Line1 Alveolar lung carcinoma bearing mice (n = 4). d. Responses against peptides of neo-antigen candidates in AE17 murine mesothelioma bearing mice (n = 5). Each dot represents mean dLN responses from an individual animal. Error bars show mean ± SD. Paired Student’s T-test.