Figure 5.

GD2-CAR-T cells prevent metastasis formation in vivo. Lungs of NSG mice injected with 5 × 10⁶ MDA-MB-231 cells into the fourth mammary fat pad on day 0, treated on day +7 with either 5 × 10⁶ CD19-CAR-T or 5 × 10⁶ GD2-CAR-T and sacrificed on day +21 were fixed and stained with the pan-cytokeratin staining AE1/3 to determine metastatic infiltration. In a), the presence of AE1/3 positive cells in the lungs of mice was quantified, 4 mice each group. In b), representative images for both groups are shown, demonstrating the presence and absence of metastatic cells.