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. 2019 Dec 10;56(2):494–507. doi: 10.3892/ijo.2019.4937

Figure 2.

Figure 2

Figure 2

Overexpression of CASC2 inhibits pancreatic cancer cell growth and progression partially by altering cell-cell adhesion. Overexpression of CASC2 suppresses (A) proliferation of AsPC-1 and PANC-1 cells, as determined by MTT assay, (B) cell colony formation of AsPC-1 and PANC-1 cells, as determined by colony formation assay, (C) migration of AsPC-1 and PANC-1 cells, as determined by wound healing assay (×40 magnification), and (D) invasion of AsPC-1 and PANC-1 cells, as determined by Transwell assay (×100 magnification). (E) Overexpression of CASC2 promotes apoptosis of AsPC-1 and PANC-1 cells, as determined by flow cytometry. (F) Western blot analysis of indicated proteins in AsPC-1 and PANC-1 cells overexpressing CASC2. (G) Morphology of AsPC-1 and PANC-1 cells transfected with CASC2 overexpression vector; scale bar, 50 µm. *P<0.05 and **P<0.01. cad, cadherin; CASC2, cancer susceptibility candidate 2; FAK, focal adhesion kinase; ITGB4, Integrin β4; MMP, matrix metalloproteinase; NC, negative control; OD, optical density; p-, phosphorylated.