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. 2019 Dec 10;56(2):494–507. doi: 10.3892/ijo.2019.4937

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-24 is a target of CASC2 in pancreatic cancer cells. (A) Predicted binding site of CASC2 and miR-24. (B) Relative luciferase activity of CASC2-WT and CASC2-MUT following transfection with miR-24 mimics or inhibitor. Luciferase activity was measured after 48 h and is presented as relative luciferase units. Expression levels of (C) CASC2 and (D) miR-24 in AsPC-1 and PANC-1 cells transfected with pIRES2-EGFP-CASC2 or empty vector. Expression levels of (E) CASC2 and (F) miR-24 in AsPC-1 and PNAC-1 cells transfected with miR-24 inhibitor or miR-24 negative control. ^*P<0.05 and ^**P<0.01. CASC2, cancer susceptibility candidate 2; miR, microRNA; MUT, mutant; NC, negative control; WT, wild type.

miR-24 is a target of CASC2 in pancreatic cancer cells. (A) Predicted binding site of CASC2 and miR-24. (B) Relative luciferase activity of CASC2-WT and CASC2-MUT following transfection with miR-24 mimics or inhibitor. Luciferase activity was measured after 48 h and is presented as relative luciferase units. Expression levels of (C) CASC2 and (D) miR-24 in AsPC-1 and PANC-1 cells transfected with pIRES2-EGFP-CASC2 or empty vector. Expression levels of (E) CASC2 and (F) miR-24 in AsPC-1 and PNAC-1 cells transfected with miR-24 inhibitor or miR-24 negative control. ^*P<0.05 and ^**P<0.01. CASC2, cancer susceptibility candidate 2; miR, microRNA; MUT, mutant; NC, negative control; WT, wild type.