Figure 1.

Effect of PIM2 knockdown on proliferation and colony formation in HepG2 and Huh-7 liver cancer cell lines. (A) WST-1 viability assay was performed at the indicated time points. A representative example of 3 independent experiments is shown. (B) Cells were transfected 48 h prior to seeding them into ultra-low attachment plates for spheroid formation. Spheroid size was documented and quantitated after 7 days (n=3). Images show representative examples. (C and D) Cells were transfected and 48 h later, colony formation was determined by performing (C) colony formation assays and (D) colony spread assays. After 7 days, the number of (distant) colonies were counted (n=4). Images show representative wells. ^*P<0.05, ^**P<0.01 and ^***P<0.001, significant differences compared to the siCtrl; the hash symbol (#) indicates that there were no significant differences. PIM2, proviral integration site for Moloney murine leukemia virus 2.