Table 1.

Displaying HPLC Parameters Investigated

Parameters Investigated	Characteristic
Column	Phenomenex Spherisorb 5 µm, 100 × 4.6 mm
Mobile Phase	Ammonium Acetate Buffer (5mM) pH 4: Acetonitrile (50:50 v/v)
Internal Standard	Caffeine
Flow Rate	1.75mL/min
Injection Volume	10µL
Column Temperature	30°C
Wavelength	225nm
Run Time	2.5 mins