Fig 5. Polystyrene nanoparticles (PS NPs) fluorescence and distribution throughout the zebrafish development.
Green fluorescence in different areas or organs of zebrafish embryos and larvae (n=5). Exposure to PS NPs started at 6 hpf and finished at 120 hpf. At this time point, PS NPs were removed and animals were allowed to depurate until 168 hpf. At 120 hpf, the yolk sac is resorbed, and the gall bladder, gastrointestinal (GI tract), pancreas, and liver are visible. Data are plotted against a logarithmic scale. Two-way ANOVA results are summarized in the bottom right panel, including the p-value for each factor (time of exposure and NP PSs concentration) and their interaction (* p<0.05, ** p<0.01, *** p< 0.001, **** p<0.0001, ns p>0.05).