Fig. 3.

The other six classes of telomere fluorescence signal patterns. DNA and telomeres are like in Fig. 2, and chr. 7 or 11 is in white on the panel below. a The one telomere incompletely removed class (“1inc.”). An orange arrow indicates left-over red fluorescence from an incompletely removed telomere strand. b The two telomeres incompletely removed class (“2inc.”). Two orange arrows indicate left-over red fluorescence from an incompletely removed telomere strand on each homolog. Both (a) and (b) are high-confidence WW:CC spreads. c The one telomere shows double fluorescence class (“1doub.”). Incomplete strand removal has led to a telomere that shows roughly equal amounts of the two fluorescence signals. This metaphase spread can only be counted as WW:CC with reduced confidence, as recombination cannot be excluded to account for this fluorescence pattern. d The one telomere shows double fluorescence, and one telomere is incompletely removed class (“1inc. and 1doub.”). A combination of (a) and (c), only low confidence in assigning WW:CC pattern. e The two telomeres show double fluorescence class (“2doub.”). Like in (c) but on both homologs, only low confidence in assigning WW:CC pattern. f The recombined class of fluorescence pattern (“Rec.”). SCE is likely to account for this fluorescence pattern, where single-stranded telomeres display identical single-colour fluorescence. Not useful for assessment of chromatid segregation patterns