Table 1.

Summary of the first round of CO-FISH experiments assessing segregation patterns of chromosomes 7 and 11 in wt ESCs)

		WW:CC								WC:WC								REC
		Ok	1inc.	2inc.	1doub.	1inc.1doub.	2doub.	High confidence	Reduced confidence	ok	1inc.	2inc.	1doub.	1inc.1doub.	2doub.	High confidence	Reduced confidence		% of total
Metaphase spreadsa
Chr. 11 (10 μm)		32	5	0	3	0	6			30	5	0	4	1	3			26
Chr. 7(10 μm)		28	10	0	1	0	6			26	6	2	4	1	5			28
Chr. 7 (125 μm)		15	13	12	3	0	4			10	11	9	3	0	2			10
Number of metaphasesb
Chr. 11 (10 μm)								37	9							35	8
Chr. 7(10 μm)								38	7							34	10		23.7
Chr. 7 (1.25 μm)								40	7							30	5		10.9

We observed random sister chromatid segregation in mitosis, both at 10 and 1.25 μM BrdU concentration

^aMetaphase spreads were subdivided into seven classes according to Fig. 2b (ok) and Fig. 3 (“one telomere with incompletely removed strand (1inc.),” “two telomeres with incompletely removed strands (2inc.),” “one telomere exhibiting double fluorescence signal (1doub.)”, one telomere with incompletely removed strand, and “one telomere exhibiting double fluorescence signal (1inc. 1doub.),” “two telomeres exhibiting double fluorescence signal (2doub.),” and “recombined (REC)”). Numbers of metaphases in each category are indicated

^bBottom: We summarized number of metaphases in high vs. reduced confidence groups. For metaphases containing recombined chromosomes 7 or 11, we calculated their overall frequency in percent. Please note that this was done for each BrdU concentration (10 vs. 1.25 μM