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. Author manuscript; available in PMC: 2020 Jan 14.
Published in final edited form as: J Immunol. 2010 Oct 1;185(9):5074–5081. doi: 10.4049/jimmunol.1001725

FIGURE 3.

FIGURE 3.

Mutation of STAT5 binding site abrogates the granulysin enhancer activity. The sequence of the STAT5 binding site construct is TTCCAGGAA. The potential STAT5 binding site TTCCAGGAA at the 59 end of the construct pGL−18,302/−18,177Mut was mutated to TGCCAGCAA using site-directed mutagenesis. After transient transfection and IL-2 stimulation, the granulysin enhancer activities of the depicted DNAs in the context of the granulysin promoter (−329 to +62) in CRL-2105 T cells or primary CD4+ T cells (as indicated) were detected as described in Fig. 2. Results are expressed as mean ± SEM. Data are representative of three independent experiments.