Fig. 2.

H&E staining of intestinal polyps after chronic zebularine treatment of Min mice. Min mice were given 0.2 mg/mL zebularine dissolved in 3% sucrose water daily for 113 ± 3 d. Mice were sacrificed at day 120. The gastrointestinal tract was removed, cut longitudinally, and washed in PBS. H&E-stained sections were observed for the presence of small polyps undetectable under a dissecting microscope. Untreated and treated males and untreated females had similarly sized polyps, but the treated females had smaller polyps at lower frequency. A, untreated male; B and C, treated male; D, untreated female; E and F, treated female.