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. Author manuscript; available in PMC: 2020 Dec 24.
Published in final edited form as: Biochemistry. 2019 Dec 11;58(51):5173–5187. doi: 10.1021/acs.biochem.9b00960

Figure 9.

Figure 9.

Orientation-selected Q-band HYSCORE spectra of dithionite-reduced globally-15N-labled D319H. The experimental spectra (contour plot) are in red, while the simulated spectra are in blue by using the parameters of g = [2.046, 1.935, 1.922]; A(15N) = [−1.02, −4.55, −1.42] MHz, Euler angle = [55, 100, 25]°.

Experimental parameters: temperature = 10 K; tπ/2 = 12 ns; tπ = 24 ns; microwave frequency = 34.161 GHz, magnetic field = 1194.4 mT, τ = 388 ns for g = 2.044; microwave frequency = 34.161 GHz, magnetic field = 1247.1 mT, τ = 372 ns for g = 1.957; microwave frequency = 34.161 GHz, magnetic field = 1269.8 mT, τ = 364 ns for g = 1.922. The time increment in both dimensions is 24 ns with 180 steps.