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. 2020 Jan 14;15(1):e0218905. doi: 10.1371/journal.pone.0218905

Fig 3. IFNAR1 and IFNAR2 mRNA expression in HIV-1-infected LPMCs.

Fig 3

LPMCs were infected with R5-tropic HIV-1NL4.3 and cultivated for 4 days. Following CD4+ T cell isolation, mRNA expression levels of IFNAR1, IFNAR2b and IFNAR2c were analyzed via qRT-PCR. Quantification of the target mRNA was normalized according to the delta-delta Ct (ΔΔCt) method and the expression fold change (2-ΔΔCt) using GAPDH as reference. The mean gene fold expression (+SEM) of each target is shown in bars (n = 4). Differences between the groups were analyzed by paired student’s t-test.