Fig 1. Nucleotide sequence alignment of the internal transcribed spacer 2 (ITS2) from B. xylophilus (Bx), B. mucronatus Europe type (BmE), B. mucronatus Asia type (BmA), B. doui (Bd), B. thailandae (Bt), and B. hylobianum (Bh).

Locations of the Bx_ITS2_F1 and Bx_ITS2_R1 primers are marked with a red- and orange-colored box, respectively. Gaps in the sequence alignment are indicated with dots. Sequence identity is indicated with blue-colored gradation.