Fig 5. The evaluation of the limit of detection (LOD) in the RPA assay for B. xylophilus.

First, a) The RPA assay used a ten-fold serial dilution of pure gDNA of B. xylophilus to measure the LOD under conditions without any interference except pure gDNA. Second, b) For the measurement for the pinewood sample LOD, RPA assay used the lysates were made by healthy pinewood mixed with the pure gDNA of B. xylophilus, which was then extracted by DAP buffer to measure the LOD under conditions with all interferences come from the pinewood with DAP buffer. P and N represent the positive control using pure gDNA from B. xylophilus and the negative control using healthy pine tree lysate extracted by DAP buffer. The line numbering is matched with absorbance numbering in each panel.