Fig 2. DSBR does not affect the cell size control of initiation of chromosomal DNA replication.
A) Distribution of the copy number of the single chromosomal origin of replication (oriC) in cells isolated from asynchronous, exponentially growing cultures of E. coli as ascertained by replication runout. Shown are representative results from three independent experiments for each strain. Data are normalized as Probability Distribution Functions (PDFs). Insert of each panel shows the estimated relative cell age at replication initiation (Ai), +/- the standard error of the mean. B) Estimated average cell length at initiation of chromosomal DNA replication calculated using the measured average copy number of oriC from three independent experiments and the estimated average cell length at birth (Fig 1B). C) C+D time (a component of the Cooper-Helmstetter model) calculated using the average mass doubling time for all strains (S1B Fig) and the average cellular oriC copy number for each strain (A). For B and C, error bars show standard error of the mean.