Fig 5. The locus undergoing DSBR does not dwell at the division plane.

A) The average cellular length at the time of lacZ replication as estimated using the average mass doubling rate (S1B Fig), the estimated cellular length at replication initiation (Fig 2B), and the rate of DNA synthesis (Fig 3C). B) Diagram of the chromosomal construct for visualizing the cellular location of lacZ. The arrays of lacO and tetO operator sites were located respectively ~6 kb on the origin-proximal and ~5 kb on the origin-distal sides of the palindrome in lacZ. LacI-CFP and TetR-YFP were expressed from a synthetic constitutive promoter integrated in the chromosome at the ykgC locus. C) Example images of lacZ localization in cells undergoing DSBR at lacZ (SbcCD⁺ lacZ::palindrome) or not (SbcCD⁺ lacZ⁺). Scale bar shows 5 μm. D) Mean number of lacZ associated LacI-CFP and TetR-YFP foci per cell. Error bars show standard error of the mean for four independent cultures. E) The spatial distribution of lacZ associated foci along the long axis of cells as a function of cell length. Each panel shows the position of lacZ-associated foci for 300 cells whose lengths lie between the estimated average length at birth and estimated average length at division for the indicated strain. For each cell, the data for LacI-CFP and TetR-YFP were aggregated.