Table 2. Summary of clinical and necropsy findings.

Fetal ID	ZF1	ZF2	ZF3	ZF4	ZF5	CF3	CF4
Dam ID	ZD1	ZD2	ZD3	ZD4	ZD5	CD3	CD4
Inoculum	ZIKV	ZIKV	ZIKV	ZIKV	ZIKV	Saline	None
GD of Inoculation	54	55	53	55	53	55	N/A
GD of Delivery	118	126	145	149	148	146	145
GD (PD) of Demise	118 (0)	128 (2)	162 (17)	166 (17)	165 (17)	167 (21)	354 (209)
Pregnancy and Neonatal Outcome	Early cervical ripening, pPROM, emergency hysterotomy with fetal euthanasia	Early cervical ripening, clinically indicated hysterotomy, neonatal death (septicemia)	Elective hysterotomy, live birth, clinically indicated euthanasia	Elective hysterotomy, live birth, experimental euthanasia	Elective hysterotomy, live birth, experimental euthanasia	Elective hyster-otomy, live birth, experimental euthanasia	Elective hyster-otomy, live birth, experimental euthanasia
Maternal Utero-placental Pathology	Small mono-discoidal placenta; multifocal infarctions, hemorrhage, villous stromal mineralization and perivasculitis	Endometritis and myometritis; plasmacytic deciduitis; villous stromal mineralization	Multifocal infarctions, acute and chronic hemorrhage, villous fibrin deposition and stromal mineralization	Multifocal infarctions, villous fibrin deposition and mineralization, neutrophilic and plasmacytic deciduitis, decidual leukocytoclastic vasculitis	Focal infarction, villous fibrin deposition and mineralization, neutrophilic and plasmacytic deciduitis, mild decidual leuko-cytoclastic vasculitis	NP	Rare stromal mineralization, focal infarction
Neonatal Clinical Presentation	N/A	Prolonged hypoxemia during resuscitation, hyperglycemia, hypotonia, bradycardia, apnea, cardio-respiratory arrest	Seizures, tachypnea, cardiomegaly, axial hypotonia, lower limb weakness, dysphagia, retarded growth	Possible mild auditory impairment complicated by presence of earwax	NSF	NSF	NSF
Fetal Gross Pathology	NSF	Cerebral meningeal and ventricular hemorrhage secondary to sepsis; small cerebellum; chin dermatitis	Cardiomegaly with mild dilation of right and left ventricles; reduced brain mass	NSF	NSF	NSF	NSF
Fetal CNS Lesions	Cerebrum—Multifocal periventricular mineralization; ependymal degeneration; increased apoptosis of neuroprogenitor cell population; increased cells noted in molecular layer of one section, presumed immature neurons (GFAP-/Iba1-/CD45-); increased astrocytes (GFAP+) in molecular layer of cingulate gyrus Cerebellum–Moderate decrease in Purkinje cell population Spinal cord—Mild to marked rarefaction and edema of white matter, thoracic cord is more affected	Multifocal hemorrhage, edema and necrosis secondary to sepsis. Pathologies atypical to sepsis cannot be conclusively attributed to Zika virus infection: Cerebrum—Periventricular mineralization, increased apoptosis of neuroprogenitor cells Cerebellum–Minimal decrease in Purkinje cell population, decreased density of external granular layer	Cerebrum—Ventricular dilation, mild ependymal loss; fewer neuroprogenitor cells Cerebellum—Attenuation of the external granular cell layer; moderate decrease in Purkinje cell population Spinal cord—Glial necrosis, white matter, minimal in lumbar cord	Cerebrum—Unilateral periventricular ependymal cell degeneration and loss, focally extensive Cerebellum—Multifocal, minimal decrease in Purkinje cell population	Cerebellum—Multifocal, moderate decrease in Purkinje cell population and decrease in external granular cell layer Spinal cord—Swollen myelin sheaths, rare gitter cells and solitary spheroid in cervical cord Dorsal root ganglion—Scattered, minimal lymphocytic infiltrates	NSF	No gross lesions; microscopic lesions not assessed
Fetal Lesions in Other Tissues	Urinary bladder—Multifocal, minimal histiocytic infiltrates	Skin of chin—Focal, mild, neutrophilic dermatitis Biceps brachii, left—Hemorrhage and edema secondary to sepsis Brachioradialis, right—Hemorrhage and edema secondary to sepsis	Heart—Cardiomyopathy, left ventricle, increased cardiomyofiber diameter, cardiomyocyte karyomegaly	Heart—Ventricle, cardiomyocyte hypertrophy and karyomegaly, rare	Heart—Multifocal, mild to moderate cardiomyocyte vacuolar degeneration and multifocal, mild karyomegaly	NSF	NSF
Fetal Post-mortem MRI	NP	T1W and T2W lesions secondary to sepsis	Reduced brain volume and surface area	NSF	NSF	NSF	NP

NSF = no significant findings, NP = not performed/not assessed, N/A = not applicable.