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. 2020 Jan 14;15(1):e0227876. doi: 10.1371/journal.pone.0227876

Fig 3. Cell viability assay of C. krusei ATCC 6258 after treatment (24 h) with LMM11 at 16 μg/mL, 32 μg/mL and 64 μg/mL.

Fig 3

In metabolically active cells, the cytoplasm presents diffusely distributed green fluorescence and contains cylindrical red fluorescent structures in vacuoles. Dead cells or cells with little or no metabolic activity present bright yellow-green fluorescence with no discernable red structures. Dead control (A1-A3): yeast treated with 70% alcohol for 15 minutes. Live control (B1-B3): untreated yeast. Brightfield images (A1, B1, C1, D1 and E1). Yeast were also stained with Calcofluor (A2, B2, C2, D2 and E2). Yeast treated with 16 μg/mL (C3) maintained a pattern of cellular metabolism similar to the live control (B3). However, 32 μg/mL (D3) and 64 μg/mL (E3) LMM11 caused a marked decrease in cell number and cell viability. The samples were observed at 400x magnification. Analysis was performed on at least 20 fields. The assays were performed the using LIVE/DEAD yeast viability kit (L7009).