Fig 5. Representative transmission electron microscopy micrographs of C. krusei ATCC 6258.

Yeast were incubated with 32 μg/mL LMM11 for 24 h at 35°C. Untreated cells had a normal appearance (A). Treated cells (B-D) presented ultrastructural alterations such as invaginated cell membrane (red arrows), irregular cell wall surfaces (black arrows), cytoplasmic retractions (asterisk), lipid vacuoles (white arrows) and cellular extravasation (yellow arrows), which were not observed in the control. The samples were observed at 25000x magnification. The analysis was performed on at least 20 fields. Bars = 0.5 μm.