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. 2020 Jan 10;99(2):e18626. doi: 10.1097/MD.0000000000018626

Figure 5.

Figure 5

Direct in vitro effects of human recombinant FABP4 (hrFABP4) and hrRBP4 on the BSA (bovine serum albumin)-induced apoptosis and corresponding signals on cultured human proximal tubule epithelial cell line HK-2 cell and human podocyte cell line. Bar graphs and IF images of the percentage of (A,C) caspase-3 (+),(E,F)TUNEL(+) [early apoptosis] and (I,J) Annexin-V+PI+ [late apoptosis] of BSA-pretreated HK-2/podocytes that concomitantly incubation with hrFABP4 (75 μg/ml) and hrRBP4 (100 μg/ml); (B,D) Proteins and (G,H) mRNA levels of ER stress and apoptosis markers in cell lysates of BSA-pretreated HK-2 and podocytes. ∗P < .05 vs untreated (unt.) group; #P < .05 vs BSA group. The results are expressed as representative of 3 independent experiments. The percentage of cell viability of each treated group compared to untreated group were calculated.