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. Author manuscript; available in PMC: 2020 Jan 14.
Published in final edited form as: Methods Mol Biol. 2018;1788:269–277. doi: 10.1007/7651_2017_78

Fig. 2.

Fig. 2

The SNaPP system was constructed based on the previously reported configuration [19]. Two Agilent 1200 series nano pumps are used to deliver the digestion buffer (Agilent-1) and SPE wash buffer (Agilent-2). (See Note 9). A Dionex UltiMate 3000 RSLCnano pump is used to deliver the RPLC separation gradient. Two 6 ports injection valves were used for loading, SNaPP samples (Vinj1), and predigested peptide samples (Vinj2). The third 6 port valve (V3) is used to set up the SPE (5 μm C18,150 μm × 4 cm) and analytical column (1.7 μm AQ C18, 50 μm × 25 cm) and one 4 port valve was used for switching between SNaPP or peptide methods (See Note 10). The system is coupled to a Thermo QExactive Mass spectrometer by a replaceable custom ESI emitter (150 μm O.D. × 20 μm I.D.); (see Notes 11 and 12)