Key resources table.
| Reagent type | Designation | Source | Identifier | Additional information |
|---|---|---|---|---|
| Strain (Escherichia coli) | BL21 (DE3)-RIL | Agilent | #230280 | |
| Chemical compound | n-Dodecyl-β-D-Maltopyranoside, Sol-Grade | Anatrace | D310S | |
| Chemical compound | n-Undecyl-β-D-Maltopyranoside, Anagrade | Anatrace | U300 | |
| Chemical compound | C8E4 | Anatrace | T350 | |
| Chemical compound | Phenanthroline | Sigma-Alrich | 131377 | |
| Commercial assay or kit | M2 agarose affinity gel | Sigma-Alrich | A4596 | |
| Commercial assay or kit | Superdex 200 increase | GE Lifesciences | 28990944 | |
| Commercial assay or kit | Glutathione Sepharose 4B resin | GE Healthcare | 17075604 | |
| Commercial assay or kit | anti-HA mouse monoclonal antibody | Invitrogen RRID:AB_10978021 |
26183 | 1:10000 |
| Commercial assay or kit | goat anti-mouse Alexa Fluor 680 | Invitrogen RRID:AB_2535723 |
A21057 | 1:1000 |
| Commercial assay or kit | M2 anti-flag mouse antibody | Sigma-Alrich | F 1804–50 UG | 1:5000 |
| Other | R1.2–1.3 400 mesh Au holey carbon grids | Quantifoil | 1210627 | |
| Software, algorithm | Leginon | RRID:SCR_016731 | http://emg.nysbc.org/redmine/projects/leginon/wiki/Leginon_Homepage | |
| Software, algorithm | Unblur | DOI:10.7554/eLife.06980 | http://grigoriefflab.janelia.org/unblur | |
| Software, algorithm | CryoSPARC | DOI: 10.1038/nmeth.4169
RRID:SCR_016501 |
https://cryosparc.com/ | |
| Software, algorithm | CTFFIND4 | DOI: 10.1016/j.jsb.2015.08.008
RRID:SCR_016732 |
http://grigoriefflab.janelia.org/ctf | |
| Software, algorithm | RELION | DOI: 10.7554/eLife.18722
DOI:10.7554/eLife.41266 RRID:SCR_016274 |
http://www2.mrc-lmb.cam.ac.uk/relion | |
| Software, algorithm | gCTF | DOI: 10.1016/j.jsb.2015.11.003
RRID:SCR_016500 |
https://www.mrc-lmb.cam.ac.uk/kzhang/ | |
| Software, algorithm | Gautomatch | https://www.mrc-lmb.cam.ac.uk/kzhang/ | ||
| Software, algorithm | alignparts_lmbfgs | DOI: 10.1016/j.jsb.2015.08.007 | https://sites.google.com/site/rubinsteingroup/direct-detector-align_lmbfgs | |
| Software, algorithm | FrealignX | DOI: 10.1016/bs.mie.2016.04.013
RRID:SCR_016733 |
http://grigoriefflab.janelia.org/frealign | |
| Software, algorithm | COOT | DOI:10.1107/S0907444910007493
RRID:SCR_014222 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |
| Software, algorithm | PHENIX | DOI: 10.1107/S0907444909052925
RRID:SCR_014224 |
https://www.phenix-online.org | |
| Software, algorithm | MolProbity | DOI: 10.1107/S0907444909042073;10.1093/nar/gkm216
RRID:SCR_014226 |
http://molprobity.biochem.duke.edu | |
| Software, algorithm | Chimera | DOI: 10.1002/jcc.20084
RRID:SCR_004097 |
https://www.cgl.ucsf.edu/chimera | |
| Software, algorithm | ChimeraX | DOI: 10.1002/pro.3235
RRID:SCR_015872 |
https://www.https://www.rbvi.ucsf.edu/chimerax/ | |
| Software, algorithm | Pymol | PyMOL RRID:SCR_000305 |
http://www.pymol.org | |
| Software, algorithm | APBS | DOI: 10.1093/nar/gkm276
RRID:SCR_008387 |
http://www.poissonboltzmann.org | |
| Software, algorithm | 3V web server | DOI: 10.1093/nar/gkq395 | http://3vee.molmovdb.org/ | |
| Software, algorithm | UniDec v 3.2 | DOI: 10.1021/acs.analchem.5b00140 | https://github.com/michaelmarty/UniDec |