Table 2. Summary of Cryo EM data and structure refinement statistics of PCAT1-CtA complex.
| Data collection | |
|---|---|
| Microscope | Titan Krios, 300 keV (FEI) |
| Detector | K2 summit direct electron detector (Gatan) |
| Energy Filter | 10 eV (Gatan) |
| Pixel size (Å) | 1.09 |
| number of movies | 3478 |
| Frames/movie | 60 |
| Total exposure time (s) | 12 |
| Exposure time per frame (s) | 0.2 |
| Total exposure (e/Å2) | 80.8 |
| Defocus range (µM) | 0.8 to 2.2 |
| Final reconstruction | |
| Number of particles | 133698 |
| B-factor correction (Å2) | −80 |
| RMS deviations | |
| bond length (Å) | 0.0028 |
| bond angles (°) | 1.2870 |
| Ramachandran | |
| favored (%) | 94.95 |
| Allowed (%) | 5.05 |
| Outlier (%) | 0.00 |
| Rotamer | |
| favored (%) | 87.30 |
| Allowed (%) | 9.73 |
| poor (%) | 2.97 |