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. 2020 Jan 14;9:e50765. doi: 10.7554/eLife.50765

Figure 8. Mk receptors are expressed throughout regenerating tissues.

(A) Heatmap of normalized transcript levels for expressed mk receptors in intact and regenerating subpopulations from our RNA-seq dataset revealed four general patterns of expression (Figure 8—source data 1). (B–C) Double RNAscope in situ hybridization of mk (bright red puncta) with its cognate receptors sdc-1 (B) or ptprz (C) (dark blue puncta). Dotted black line denotes wound epidermis/AEC boundary. Since ptprz expression was low during early stages of regeneration, ptprz/mk in situs were performed without a hematoxylin counterstain to ease visualization of dark blue puncta in the wound epidermis at 7 dpa. Scale bars, B and C (left panel): 25 µm for 63x magnification, C (right panel): 50 µm for 40x magnification. WE, wound epidermis, AEC, apical epithelial cap, bl, blastema, dpa, days post-amputation.

Figure 8—source data 1. TPM values and transcript IDs for midkine receptors in Tsai et al. (2019).

Figure 8.

Figure 8—figure supplement 1. Mk receptors are not highly expressed in myeloid cells during wound healing.

Figure 8—figure supplement 1.

Publicly available single cell expression data of mk receptors from Leigh et al. (2018). Top left panel shows original clustering of single cell expression profiles at the wound healing stage of blastemal progenitors, wound epidermis, and myeloid cell populations circled. Top right panel shows t-SNE plot of apoeb expression, which was identified as highly enriched in myeloid cells, to give an example of a highly expressed gene in the myeloid cell populations. All panels below are t-SNE plots showing expression of mk receptors.