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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Metab Eng. 2019 Nov 28;57:193–202. doi: 10.1016/j.ymben.2019.11.009

Figure 3.

Figure 3.

Engineered E. coli strains that directly produce MPLA. (a) Schematic illustration of the construction of the MPLA-producing E. coli strain KHSC0003/pBAD33.1-lpxEs. (b) Schematic diagram of the MPLA-producing strain. (c) Growth curves for all engineered strains and the mother strain W3110. The number 1–7 correspond to the strains shown on panel (a). (d) TLC profiles of the lipids extracted from the engineered E. coli strains compared to those of GLA (lane 4) and MPLAsm (lane 5). Total lipids extracted from KHSC0003/pBAD33.1 (lane 1), KHSC0003/pBAD33.1-lpxEAA (lane 2), and KHSC0003/pBAD33.1-lpxEHP (lane 3).