Table 1.
Strains and plasmids used in this study
| Strain | Relevant properties | Source or reference |
|---|---|---|
| W3110 | Wild type F− λ− rph-1 IN(rmD, rmE) | E. coli Genetic Stock Center, Yale |
| C41(DE3) | F− ompT hsdSB (rB−mB−) gal dcm (DE3) F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 | [42] |
| DH5α | endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 relA1 λ− | [18] |
| JW2162 | lpxT::kan BW25113 | [26] |
| JW0617 | pagP::kan BW25113 | [26] |
| HSC1/pEcKdtA | kdtA::kan DY330/pEcKdtA | [22] |
| KIHSC001 | lpxT::kan W3110 | This work |
| KIHSC002 | ΔlpxT W3110 | This work |
| KIHSC003 | ΔlpxT pgpP::kan W3110 | This work |
| KHSC0001 | ΔlpxT ApagP W3110 | This work |
| KHSC0001–1 | KHSC0001/pWSK29-lpxLEClpxMEC | This work |
| KHSC0003 | kdtA::kan KHSC0001–1 | This work |
| KHSC0003–1 | KHSC0003/pBAD33.1 | This work |
| KHSC0003–2 | KHSC0003/pBAD33.1-lpxEAA | This work |
| KHSC0003–3 | KHSC0003/pBAD33.1-lpxEHP | This work |
| KHSC0003–4 | KHSC0003/pBAD33.1-lpxEFN | This work |
| KHSC0003–5 | KHSC0003/pBAD33.1-lpxERL | This work |
| Plasmid | Relevant properties | Reference |
| pWSK29 | Low copy vector, lac promoter, AmpR | [23] |
| pWSK29-lpxLEclpxMEc | pWSK29 harboring E. coli lpxL and lpxM | This work |
| pET21a-lpxEAA | pET21a harboring A. aeolicus lpxE (aq_1706) | [19] |
| pBAD33.1 | Medium copy vector, CamR | [22] |
| pBAD33.1-lpxEAA | pBAD33.1 harboring A. aeolicus lpxE (aq_1706) | This work |
| pBAD33.1-lpxEHP | pBAD33.1 harboring H. pyrolilpxEHP(hp0021) | This work |