Table 2.
Mutation analyses of KHSC0003/pBAD33.1-lpxEAA genome via de novo sequencing in comparison with the published W3110 genome (Genbank accession number: AP009048) and that of the mother strain W3110 used for the strain construction.
| Nucleotide position based on AP009048 | W3110 (AP009048) | W3110 (mother strain) | KHSC0003/pBAD33.1-lpxEAA |
|---|---|---|---|
| 151,154 | C | C | G (gly149ala mutation YadK) |
| 547,694 | A | G | G |
| 547,831 | AGGGG | AGGGGG | AGGGGG |
| 556,858 | A | T | T |
| 1,065,700 | C | T | T |
| 1,093,686 | T | C | C |
| 1,097,580 | C | T | T |
| 1,117,836 | C | C | C |
| 1,303,712 | A | G | G |
| 2,019,202 | C | T | T |
| 2,906,975 | C | G | G |
| 4,371,270 | GAAAAA | GAAA | GAAA |
| 653,806 – 656,391 | IS elements inserted in dcuC | Intact ducC | Intact ducC |
| 656,979 – 657,539 | pagP | pagP | Deleted |
| 2,272,188 – 2,272,901 | lpxT | lpxT | Deleted |
| 3,830,597 – 3,831,875 | kdtA | kdtA | Replaced with a kanamycin resistance cassette |