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Batch-to-batch variations in EcML yield and congener composition.
| Batch | Yield mg/25g LB (mg/g DCWa) | Composition (%): number of acyl chain | |||
|---|---|---|---|---|---|
| Hexa | Penta | Tetra | |||
| EcML | 1 | 8.4 (12.7) | 68.5 | 23.7 | 7.9 |
| 2 | 12.4 (18.6) | 66.6 | 24.5 | 9.0 | |
| 3 | 8.4 (13.9) | 73.9 | 18.5 | 7.6 | |
| Average ± STD | 9.7 ± 2.3 (14.7 ± 3.3) | 69.7 ± 3.8 | 22.2 ± 3.3 | 8.2 ± 0.7 | |
DCW of KHSC0003/pBAD33.1-lpxEAA is 0.29 g for 1L of OD600 = 1.0
