Table 2. Cryoprotective Outcomes Using Polyampholytes.

Structurea	[Polymer] (wt %)	Cell type	Cell viabilityb	Freezing ratec	Other CPAsd	Ref
2	7	L929	∼95%	Slow freezing 1 °C·min–1	None	(60)
3	15	L929	∼90%	Slow freezing NCe	None	(62)
4	10	L929	∼95%	Slow freezing NCe	None	(63)
5	15		<10%
6	15		∼70%
7	2	RBC	∼65%	Vitrification direct into LN2	350 mg·mL–1 HES, 30 mg·mL–1 mannitol and 6.5 mg·mL–1 NaCl	(65)
8	12	L929	∼90%	Slow freezing NCe	None	(87)
2	10	MSC Monolayer	∼80%	Slow freezing 4.9 °C·min–1	6.5 M EGf, 0.5 M sucrose	(25)
3	10	L929	∼60%	Slow freezing NCe	None	(61)
9	10		∼70%
4	10		96%
10	10	3T3	70%	Slow freezing 1 °C·min–1	2% DMSO	(83)
11	10		90%
2	20	PN Pig embryo	Higher development rate	Vitrification direct into LN2	30% EGf, 0.5 M sucrose	(91)
2	10	Chondrocyte sheet	All sheets recovered	Vitrification direct into LN2	20% DMSO, 20% EGf, 0.5 M sucrose	(89)
2	10	Mouse oocyte	95% Survival after fertilization	Vitrification direct into LN2	20% EGf, 0.5 M sucrose	(90)
2	7.5	Human mesenchymal stem cells	90% Viability after 24 months	Slow freezing NCe	None	(93)
1	2	A549 Suspension	50%	Slow freezing 1 °C·min–1	5% DMSO	(24)
1	4	A549 Monolayer	90%	Slow freezing 1 °C·min–1	5% DMSO

^aRepresentative structure reproduced from reference.

^bViability of the cells as reported.

^cIf included in original ref.

^dAny other materials added to the cryopreservation solution.

^eNot controlled, final storage temperature −80 °C.

^fEG is ethylene glycol.