Change of protein profile in the pollen extracts over 1 year of storage in various conditions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of ragweed extract reconstituted in normal saline (NS), in 50% glycerol, in phenol saline in 10% glycerol, and in phenol saline in 50% glycerol kept at room temperature (RT) (A); ragweed in NS, ragweed in 50% glycerol, ragweed in phenol saline in 10% glycerol, ragweed phenol saline in 50% glycerol at 4°C (B); mugwort in NS, in 50% glycerol, in phenol saline in 10% glycerol, and in phenol saline in 50% glycerol at RT (C); mugwort in NS, in 50% glycerol, in phenol saline in 10% glycerol, and in phenol saline in 50% glycerol at 4°C (D); Japanese hop in NS, in 50% glycerol, in phenol saline in 10% glycerol, in phenol saline in 50% glycerol at RT (E); Japanese hop in NS, in 50% glycerol, in phenol saline in 10% glycerol, and in phenol saline in 50% glycerol at 4°C (F); sawtooth oak in NS, in 50% glycerol, in phenol saline in 10% glycerol, in phenol saline in 50% glycerol at RT (G); sawtooth oak in NS, in 50% glycerol, in phenol saline in 10% glycerol, and in phenol saline in 50% glycerol at 4°C (H).