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. 2020 Jan 15;12(1):66–76. doi: 10.4251/wjgo.v12.i1.66

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©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Rat livers with lipid accumulation and circulating lipid levels. A: The livers of control rats with normal diet; B: The livers of the rats with high fat diet; C: The livers of the rats with high fat plus 2-fluorenylacetamide (2-FAA) diet at the early stage; D: The livers of the rats with high fat plus 2-FAA diet at the middle early stage; and E: The livers of the rats with high fat plus 2-FAA diet at the last stage; A1: Normal controls; B1-E1: The sections of the corresponding to above livers were stained with the Oil red O assay, and over lipid accumulation in rat hepatocytes; F: The integral optic density values represented hepatic lipid levels of the corresponding to above livers; G: The alterations of serum total cholesterol level; and H: The alterations of serum triglycerides level. Original magnification of liver sections (× 400) from Figure 1A1 to Figure 1E1. ^aP < 0.05 vs control group. IOD: Integral optic density; Tch: Total cholesterol; TG: Triglycerides.