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. 2020 Jan 8;10:1552. doi: 10.3389/fphys.2019.01552

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Copyright © 2020 Ishikawa, Fujiwara, Wong, Ura and Kamikouchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Labeled JO neurons in a scolopidium. (A) The antennal ear of fruit flies. Johnston’s organ (JO) is housed in the second antennal segment. (B) Scolopidium in the JO. A schema of a section at the dashed horizontal line is shown in (C). (C) Horizontal view of a scolopidium. Labeled cilia are visualized as dots (green) in a scolopidium (magenta). (D,E) Labeled JO neurons in GAL4 driver strains that label all JO subgroup neurons. Scolopidia containing JO1- (D) and elav^c155-labeled cilia (E). Arrows indicate the examples of labeled cilia (green) in a scolopidium (magenta) (left panels). Dotted square indicates the area that is magnified in the right panel. Arrowheads and asterisks in the magnified views indicate the selected and not-selected scolopidia, respectively (right panels). Scale bar = 10 μm. Panels (A) and (B) were modified from Ishikawa and Kamikouchi (2016) and Matsuo et al. (2016) with permissions.