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. 2020 Jan 8;10:2962. doi: 10.3389/fmicb.2019.02962

FIGURE 2.

FIGURE 2

CSFV infection promotes ubiquitination of Parkin2 and mediates NDP52 modification via the PINK1-Parkin pathway. (A) PK-15 cells were treated as in Figure1 A. Western blotting was used to examine Parkin2, Npro and GAPDH protein expression 24, 36, and 48 h after CSFV infection. (B) PK-15 cells were infected with CSFV (MOI = 0.1) for 24 h. Cell lysates were immunoprecipitated with an anti-Parkin antibody and assayed for Parkin2 with anti-Ub(A-5) antibody. (C) PK-15 cells were infected with CSFV (MOI = 0.1) for 24 h, followed by shParkin2 or shNC plasmid (MOI = 10) for 24 h. After immunoprecipitation with an anti-NDP52 antibody, NDP52 ubiquitination and SUMOylation were blotted with anti-Ub-5a and anti-SUMO2-4 antibodies.