Table 3.
WB working antibody dilutions.
| WB markers | Catalog number | Dilution |
|---|---|---|
| Anti-IL1β, clone B122 | sc-12742 (Santa Cruz, Biotech.) | 1:1,000 |
| Biotin anti-IFNγ | 505803 (BioLegend) | 1:1,000 |
| Biotin anti-IL10 | 554465 (BD Biosciences) | 1:500 |
| Biotin anti-TGFβ | 555053 (BD Biosciences) | 1:500 |
| Anti-β-actin | 622101 (BioLegend) | 1:500 |
The corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Jackson ImmunoResearch. Streptavidin-horseradish peroxidase (SAv-HRP) was purchased from BioLegend and used for biotin-tag detection. Loading control: β-actin.