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Viability of fresh, frozen, and melatonin-treated ram sperm (n = 10). C was fresh sperm, F was frozen-thawed sperm, F+M (red column) was frozen-thawed sperm with melatonin. Viability was determined by PI staining with flow cytometry (A–C), the percentage of fluorescent sperm in of fresh, frozen, and melatonin-treated ram sperm (D). The experiment was repeated three times. *P < 0.05.
