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Concentrations of CS, IDH, OGDC, and F0F1-ATP synthase in fresh, frozen, and melatonin-treated ram sperm (n = 10). C was fresh sperm, F was frozen-thawed sperm, F+M (red column) was frozen-thawed sperm with melatonin. TCA, tricarboxylic acid cycle; CS, citrate synthase; IDH, isocitrate dehydrogenase; OGDC, 2-Oxoglutarate dehydrogenase complex. The experiment was repeated three times. *P < 0.05.
