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. 2020 Jan 8;10:787. doi: 10.3389/fendo.2019.00787

Table 3.

Ligand binding and cross reactivity of monoclonal antibodies used in 3,5-T2 and 3-T1AM CLIA as analyzed by immune extraction.

mAb 2H4-3-T1AM* 13C9-15N-3,5-T2* 3,5-T2 T3 rT3 T4 3-T1-AM
(3-T1-AM) graphic file with name fendo-10-00787-i0001.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0001.jpg
(3,5-T2) graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0001.jpg graphic file with name fendo-10-00787-i0001.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg graphic file with name fendo-10-00787-i0002.jpg

Ligand binding specificity and cross reactivity with other thyroid hormones/metabolites was analyzed by immune extraction from native and spiked serum in parallel using monoclonal antibodies (mAb) employed in the CLIA immune assays. Extracts bound to the mAb were subsequently analyzed by LC-MS-MS. No significant cross reactivity for the analytes indicated was detectable.

*

Stable-isotopically-labeled THM used as internal standards in LC-MS/MS.