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. 2020 Jan 8;13:568. doi: 10.3389/fncel.2019.00568

Figure 2.

Figure 2

Inhibition of the mevalonate pathway by HMGCS-1 siRNAs or simvastatin reduces the UPRmt reaction induced by Aβ25–35 in SHSY5Y cells. (A) Western blot analysis of HMGCS-1 protein level in SHSY5Y cells treated with 20 μM Aβ25–35 for 4 h, quantification and β-Actin served as the internal control. The data are mean ± SEM (n = 3, *p < 0.05 vs. control group). (B) Western blot analysis of HMGCS-1 and UPRmt related proteins level in SHSY5Y cells transfected with scramble or HMGCS-1 siRNAs for 48 h, quantification and β-Actin served as the internal control. The data are mean ± SEM (n = 3, *p < 0.05 vs. siNC group). (C) Western blot analysis of HMGCS-1 and UPRmt related proteins level in SHSY5Y cells transfected with scramble or HMGCS-1 siRNAs for 48 h and 20 μM Aβ25–35 treatment for 4 h, quantification and β-Actin served as the internal control. The data are mean ± SEM (n = 3, *p < 0.05 vs. siNC group). (D) After treated with 1 μM and 10 μM simvastatin for different time courses, we detected the UPRmt related proteins level in SHSY5Y cell with 20 μM Aβ25–35 by western blot analysis, β-Actin served as the internal control.The data are mean ± SEM (n = 3, *p < 0.05 vs. DMSO group).