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Enforced expression of Krüppel-like factor 4 (KLF4) in the lung attenuated MET in vivo. Triple-labeled immunofluorescence staining were performed to examine the expression of KLF4 (red), E-cadherin (green), and vimentin (pink) in mouse lungs. (A–F) Typical images of control AAV5 vector group, and (G–L) typical images of AAV5 expressing KLF4 group. (M) The number of triple-labelled positive cells was calculated in each group. The values were shown as mean ± SD. **P < 0.05 by t-test.
