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. 2020 Jan 8;10:1512. doi: 10.3389/fphar.2019.01512

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Copyright © 2020 Wu, Lin, Wang, Li, Liu, Ye, Huang, Lin, Liu, Zhang, Li, Zhao, Lv, Li, Wang and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ectopic expression of Krüppel-like factor 4 (KLF4) suppressed lung cancer cell migration. (A–D). (A) H1299, (B) H1650, (C) A549, and (D) H226 lung cancer cells were subjected to a wound-healing assay. Representative areas of migrated cells and wound closing area (%) are shown (mean ± SEM). **p < 0.05. The experiment was repeated at least three times. Representative photographs were taken at 4× magnification. AdKLF4, Lung cancer cells were transfected with adenovirus expressing KLF4. AdKLF4+Tc, Lung cancer cells were transfected with adenovirus expressing KLF4 and cultured with tetracycline.